Dysferlin Antibody (3U1P9)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15781

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15781-20ul
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 3U1P9

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Dysferlin Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 50-150 of human Dysferlin (O75923). FEWDLKGIPLDQGSELHVVVKDHETMGRNRFLGEAKVPLREVLATPSLSASFNAPLLDTKKQPTGASLVLQVSYTPLPGAVPLFPPPTPLEPSPTLPDLDV

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Dysferlin Antibody (3U1P9)

Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781]

Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781]

Western Blot: Dysferlin Antibody (3U1P9) [NBP3-15781] - Western blot analysis of extracts of RD cells, using Dysferlin Rabbit mAb (NBP3-15781) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: Dysferlin Antibody (3U1P9) [NBP3-15781]

Immunohistochemistry-Paraffin: Dysferlin Antibody (3U1P9) [NBP3-15781]

Immunohistochemistry-Paraffin: Dysferlin Antibody (3U1P9) [NBP3-15781] - Immunohistochemistry of paraffin-embedded human placenta using Dysferlin Rabbit mAb (NBP3-15781) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Applications for Dysferlin Antibody (3U1P9)

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dysferlin

Dysferlin is the protein product of the 2p13 gene that is defective in patients with Limb-Girdle Muscular Dystrophy type 2B (LGMD2B) and Miyoshi Myopathy (MM). Dysferlin is normally localized to the muscle plasma membrane. In patients with LGMD2B and MM, immunoreactivity to dysferlin is severely reduced or lost, depending on the type of mutation. This antibody is used for the characterization of LGMD2B and MM.

Alternate Names

dysferlin, dysferlin, limb girdle muscular dystrophy 2B (autosomal recessive), dystrophy-associated fer-1-like 1, FER1L1Dystrophy-associated fer-1-like protein, Fer-1-like protein 1, FLJ00175, FLJ90168, LGMD2B

Gene Symbol

DYSF

Additional Dysferlin Products

Product Documents for Dysferlin Antibody (3U1P9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Dysferlin Antibody (3U1P9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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