Dystonin Antibody (1B10)

Novus Biologicals, part of Bio-Techne | Catalog # H00000667-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human, Mouse

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1B10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Dystonin Antibodies »

Product Specifications

Immunogen

DST (NP_899236, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMIS

Specificity

DST - dystonin

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Dystonin Antibody (1B10)

Immunocytochemistry/ Immunofluorescence: Dystonin Antibody (1B10) [H00000667-M01]

Immunocytochemistry/ Immunofluorescence: Dystonin Antibody (1B10) [H00000667-M01]

Immunocytochemistry/Immunofluorescence: Dystonin Antibody (1B10) [H00000667-M01] - Analysis of monoclonal antibody to DST on HeLa cell . Antibody concentration 10 ug/ml.
ELISA: Dystonin Antibody (1B10) [H00000667-M01]

ELISA: Dystonin Antibody (1B10) [H00000667-M01]

ELISA: Dystonin Antibody (1B10) [H00000667-M01] - Detection limit for recombinant GST tagged DST is approximately 0.1ng/ml as a capture antibody.

Applications for Dystonin Antibody (1B10)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein with GST tag on ELISA and WB. GST tag alone is used as a negative control. It has been use for IF.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dystonin

This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been known that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration.

Alternate Names

230 kDa bullous pemphigoid antigen, BP240, BPAFLJ30627, BPAG1FLJ32235, Bullous pemphigoid antigen, Bullous pemphigoid antigen 1, bullous pemphigoid antigen 1, 230/240kDa, CATX-15, D6S1101, DKFZp564B2416, DMHCATX15, DT, Dystonia musculorum protein, dystonin, FLJ13425, FLJ21489, FLJ46791, Hemidesmosomal plaque protein, KIAA0465, KIAA0728, KIAA1470, MACF2, trabeculin-beta

Entrez Gene IDs

667 (Human)

Gene Symbol

DST

OMIM

113810 (Human)

UniProt

NP_899236 (Human)

Additional Dystonin Products

Product Documents for Dystonin Antibody (1B10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dystonin Antibody (1B10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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