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Dystonin Antibody (1D2)

Novus Biologicals, part of Bio-Techne | Catalog # H00000667-M02

Novus Biologicals, part of Bio-Techne
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H00000667-M02

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 1D2

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

DST (NP_899236, 401 a.a. ~ 500 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMIS

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for Dystonin Antibody (1D2)

Immunocytochemistry/ Immunofluorescence: Dystonin Antibody (1D2) [H00000667-M02]

Immunocytochemistry/ Immunofluorescence: Dystonin Antibody (1D2) [H00000667-M02]

Immunocytochemistry/Immunofluorescence: Dystonin Antibody (1D2) [H00000667-M02] - Analysis of monoclonal antibody to DST on HeLa cell . Antibody concentration 10 ug/ml.
Sandwich ELISA: Dystonin Antibody (1D2) [H00000667-M02] - Detection limit for recombinant GST tagged DST is 0.1 ng/ml as a capture antibody.

Applications for Dystonin Antibody (1D2)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
It has been used for ELISA and WB.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Dystonin

This gene encodes a member of the plakin protein family of adhesion junction plaque proteins. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene, but the full-length nature of some variants has not been defined. It has been known that some isoforms are expressed in neural and muscle tissue, anchoring neural intermediate filaments to the actin cytoskeleton, and some isoforms are expressed in epithelial tissue, anchoring keratin-containing intermediate filaments to hemidesmosomes. Consistent with the expression, mice defective for this gene show skin blistering and neurodegeneration.

Alternate Names

230 kDa bullous pemphigoid antigen, BP240, BPAFLJ30627, BPAG1FLJ32235, Bullous pemphigoid antigen, Bullous pemphigoid antigen 1, bullous pemphigoid antigen 1, 230/240kDa, CATX-15, D6S1101, DKFZp564B2416, DMHCATX15, DT, Dystonia musculorum protein, dystonin, FLJ13425, FLJ21489, FLJ46791, Hemidesmosomal plaque protein, KIAA0465, KIAA0728, KIAA1470, MACF2, trabeculin-beta

Gene Symbol

DST

Additional Dystonin Products

Product Documents for Dystonin Antibody (1D2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Dystonin Antibody (1D2)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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