Dystrobrevin beta Antibody (OTI3A12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-01798

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI3A12

Concentration

0.71 mg/ml

View all Dystrobrevin beta Antibodies »

Product Specifications

Immunogen

Full length human recombinant protein of human DTNB(NP_899205) produced in HEK293T cell.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

63.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Dystrobrevin beta Antibody (OTI3A12)

Western Blot: Dystrobrevin beta Antibody (OTI3A12) [NBP2-01798]

Western Blot: Dystrobrevin beta Antibody (OTI3A12) [NBP2-01798]

Western Blot: Dystrobrevin beta Antibody (3A12) [NBP2-01798] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Dystrobrevin beta (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Dystrobrevin beta.
Immunocytochemistry/Immunofluorescence: Dystrobrevin beta Antibody (3A12) [NBP2-01798] Staining of COS7 cells transiently transfected by pCMV6-ENTRY Dystrobrevin beta.
Immunohistochemistry-Paraffin: Dystrobrevin beta Antibody (OTI3A12) [NBP2-01798]

Immunohistochemistry-Paraffin: Dystrobrevin beta Antibody (OTI3A12) [NBP2-01798]

Immunohistochemistry-Paraffin: Dystrobrevin beta Antibody (3A12) [NBP2-01798] - Staining of paraffin-embedded Human prostate tissue using anti-Dystrobrevin beta mouse monoclonal antibody.
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Applications for Dystrobrevin beta Antibody (OTI3A12)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.71 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Dystrobrevin beta

This gene encodes dystrobrevin beta, a component of the dystrophin-associated protein complex (DPC). The DPC consists of dystrophin and several integral and peripheral membrane proteins, including dystroglycans, sarcoglycans, syntrophins and dystrobrevin alpha and beta. The DPC localizes to the sarcolemma and its disruption is associated with various forms of muscular dystrophy. Dystrobrevin beta is thought to interact with syntrophin and the DP71 short form of dystrophin. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq].

Alternate Names

beta-dystrobrevin, DTN-B, dystrobrevin beta, dystrobrevin, beta, MGC17163, MGC57126

Entrez Gene IDs

1838 (Human)

Gene Symbol

DTNB

Additional Dystrobrevin beta Products

Product Documents for Dystrobrevin beta Antibody (OTI3A12)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Dystrobrevin beta Antibody (OTI3A12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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