E. Coli Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB200-579

Novus Biologicals, part of Bio-Techne
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NB200-579
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Key Product Details

Species Reactivity

Validated:

Mouse, Non-species specific

Cited:

Mouse, Bacteria, Bacteria - Escherichia coli

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

4.5 mg/ml

View all E. Coli Antibodies »

Product Specifications

Immunogen

Mixture of E. coli serotypes.

Reactivity Notes

Cross-reacts with related Enterobacteriaceae. Mouse reactivity reported in multiple pieces of scientific literature.

Localization

Whole cell

Specificity

Reacts with all "O" and "K" antigenic serotypes of Escherichia coli. Antiserum is not absorbed for other members of the Enterobacteriaciae. Will remove E. coli proteins from recombinant preparations.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for E. Coli Antibody

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500
Application Notes
Use in IHC-Frozen reported in scientific literature (PMID: 27214553, 27122612). Use in IHC reported in scientific literature (PMID 27732661).

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

10mM PBS (pH 7.2)

Preservative

0.1% Sodium Azide

Concentration

4.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E. Coli

Escherichia coli, commonly referred to as E. coli, is a bacteria found in the lower intestine of warm-blooded organisms. While some strains are benign, others may cause serious food poisoning in humans. Virulent strains of E. coli can cause gastroenteritis, urinary tract infections, or neonatal meningitis, as well as a host of other rarer conditions. E. coli has become very important in research due to its ability to be easily manipulated. Recombinant proteins may be produced using E. coli. Since the first application of recombinant DNA technology, E. coli has been used to produce human insulin, vaccines, immobilized enzymes, and more.

Alternate Names

Escherichia coli

Additional E. Coli Products

Product Documents for E. Coli Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for E. Coli Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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