E-Selectin/CD62E Antibody (RM0034-9J12) [PE/Atto594]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-85473PEATT594

Novus Biologicals, part of Bio-Techne
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NB110-85473PEATT594
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Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

PE/Atto594 (Excitation = 488 nm, Emission = 627 nm)

Antibody Source

Monoclonal Rat IgG2 Clone # RM0034-9J12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E-Selectin/CD62E Antibodies »

Product Specifications

Immunogen

Cytokine-stimulated mouse E-Selectin/CD62E

Clonality

Monoclonal

Host

Rat

Isotype

IgG2

Applications for E-Selectin/CD62E Antibody (RM0034-9J12) [PE/Atto594]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: E-Selectin/CD62E

CD62E (also known as E-selectin, ELAM-1, and LECAM-1) is a 115 kD type I membrane protein and a member of the selectin family. CD62E is highly expressed on activated endothelial cells (IL-2, TNF- alpha, other cytokines can increase expression) and can also be expressed on endothelial cells in the skin, bone marrow and placenta. CD62E is involved in tethering and leucocyte rolling on activated endothelium at inflammatory sites and may also play a role in tumor metastasis and angiogenesis. CD62E binds to both Siayl Lewis X (CD15s) and PSGL-1 (CD162). The HCD62E antibody has been shown to recognize human CD62E and to be useful for flow cytometry.

Alternate Names

CD62E, ELAM1, LECAM2, SELE

Gene Symbol

SELE

Additional E-Selectin/CD62E Products

Product Documents for E-Selectin/CD62E Antibody (RM0034-9J12) [PE/Atto594]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E-Selectin/CD62E Antibody (RM0034-9J12) [PE/Atto594]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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