E2F-1 Antibody (16G7)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-210
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 16G7
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
The region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
Reactivity Notes
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.
Specificity
This is specific for human E2F-1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
47 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for E2F-1 Antibody (16G7)
![Western Blot: E2F-1 Antibody (16G7) [NB600-210]](https://resources.bio-techne.com/images/products/E2F-1-Antibody-16G7-Western-Blot-NB600-210-img0011.jpg "Western Blot: E2F-1 Antibody (16G7) [NB600-210]")
Western Blot: E2F-1 Antibody (16G7) [NB600-210]
Western Blot: E2F-1 Antibody (16G7) [NB600-210] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with E2F1 antibody [16G7] diluted at 1:1000. The signal was developed with Trident ECL plus-Enhanced.![Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210]](https://resources.bio-techne.com/images/products/E2F-1-Antibody-16G7-Immunocytochemistry-Immunofluorescence-NB600-210-img0010.jpg "Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210]")
Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210]
Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (16G7) [NB600-210] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: E2F1 protein stained by E2F1 antibody [16G7] diluted at 1:200. Red: phalloidin, a cytoskeleton marker, diluted at 1:50.Applications for E2F-1 Antibody (16G7)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 - 1:1000
Immunohistochemistry
1:100-1:1000
Immunohistochemistry-Paraffin
1:100 - 1:1000
Western Blot
1:500 - 1:3000
Application Notes
IP-Assay dependent
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: E2F-1
Long Name
E2F Transcription Factor 1
Alternate Names
E2F1, PBR3, RBAP-1, RBBP-3, RBP3
Entrez Gene IDs
1869 (Human)
Gene Symbol
E2F1
Additional E2F-1 Products
Product Documents for E2F-1 Antibody (16G7)
Product Specific Notices for E2F-1 Antibody (16G7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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