E2F-1 Antibody (5Z5U3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15785

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15785-100ul
NBP3-15785-20ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5Z5U3

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all E2F-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 338-437 of human E2F-1 (Q01094). PPSSLTTDPSQSLLSLEQEPLLSRMGSLRAPVDEDRLSPLVAADSLLEHVREDFSGLLPEEFISLSPPHEALDYHFGLEEGEGIRDLFDCDFGDLTPLDF

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E2F-1 Antibody (5Z5U3)

Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785]

Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785]

Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Western blot analysis of extracts of rat spleen, using E2F-1 antibody (NBP3-15785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1min.
Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785]

Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785]

Western Blot: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Western blot analysis of extracts of various cell lines, using E2F-1 antibody (NBP3-15785) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunoprecipitation: E2F-1 Antibody (5Z5U3) [NBP3-15785]

Immunoprecipitation: E2F-1 Antibody (5Z5U3) [NBP3-15785]

Immunoprecipitation: E2F-1 Antibody (5Z5U3) [NBP3-15785] - Immunoprecipitation analysis of 300ug extracts of HT-29 cells using 3ug E2F-1 antibody (NBP3-15785). Western blot was performed from the immunoprecipitate using E2F-1 antibody (NBP3-15785) at a dilition of 1:1000.

Applications for E2F-1 Antibody (5Z5U3)

Application
Recommended Usage

Immunoprecipitation

1:50 - 1:100

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: E2F-1

E2F1 is encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Long Name

E2F Transcription Factor 1

Alternate Names

E2F1, PBR3, RBAP-1, RBBP-3, RBP3

Gene Symbol

E2F1

Additional E2F-1 Products

Product Documents for E2F-1 Antibody (5Z5U3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E2F-1 Antibody (5Z5U3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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