E2F-1 Antibody (S03-5G3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-19622

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-19622-100ul
NBP3-19622-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # S03-5G3

Concentration

0.3 mg/ml

View all E2F-1 Antibodies »

Product Specifications

Immunogen

A synthetic peptide of human E2F-1 (Uniprot # Q01094)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for E2F-1 Antibody (S03-5G3)

Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622]

Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622]

Western Blot: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Western blot detection of E2F-1 in C6, 3T3 cell lysates using NBP3-19622 (1:1000 diluted). Predicted band size: 47kDa. Observed band size: 70kDa.
Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622]

Immunocytochemistry/ Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622]

Immunocytochemistry/Immunofluorescence: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunofluorescence of E2F-1 (green) in Hela cells using NBP3-19622 at dilution 1/50, and DAPI (blue)
Immunohistochemistry-Paraffin: E2F-1 Antibody (S03-5G3) [NBP3-19622]

Immunohistochemistry-Paraffin: E2F-1 Antibody (S03-5G3) [NBP3-19622]

Immunohistochemistry-Paraffin: E2F-1 Antibody (S03-5G3) [NBP3-19622] - Immunohistochemistry of E2F-1 in paraffin-embedded Human tonsil using NBP3-19622 at dilution 1/2

Applications for E2F-1 Antibody (S03-5G3)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1/50

Immunohistochemistry

1/20

Western Blot

1/1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA

Preservative

0.01% Sodium Azide

Concentration

0.3 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: E2F-1

E2F1 is encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Long Name

E2F Transcription Factor 1

Alternate Names

E2F1, PBR3, RBAP-1, RBBP-3, RBP3

Gene Symbol

E2F1

Additional E2F-1 Products

Product Documents for E2F-1 Antibody (S03-5G3)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E2F-1 Antibody (S03-5G3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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