E4F1 Antibody (PCRP-E4F1-2D1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07269

Novus Biologicals, part of Bio-Techne
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NBP3-07269-100ug
NBP3-07269-20ug
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Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Protein Array

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # PCRP-E4F1-2D1

Concentration

0.2 mg/ml

View all E4F1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human E4F1 protein (Uniprot: Q66K89)

Localization

Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

83 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08349)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for E4F1 Antibody (PCRP-E4F1-2D1)

Immunocytochemistry/ Immunofluorescence: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269]

Immunocytochemistry/ Immunofluorescence: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269]

Immunocytochemistry/Immunofluorescence: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Immunofluorescence Analysis of PFA-fixed MCF7 cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti- Mouse IgG-CF488 (green); counterstain phalloidin (red).
Flow Cytometry: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269]

Flow Cytometry: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269]

Flow Cytometry: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Flow Cytometric Analysis of PFA-fixed HeLa cells. E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1) followed by goat anti- Mouse IgG-CF488 (blue); unstained cells (red).
Protein Array: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269]

Protein Array: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269]

Protein Array: E4F1 Antibody (PCRP-E4F1-2D1) [NBP3-07269] - Analysis of Protein Array containing more than 19,000 full-length human proteins using E4F1 Mouse Monoclonal Antibody (PCRP-E4F1-2D1).

Applications for E4F1 Antibody (PCRP-E4F1-2D1)

Application
Recommended Usage

Flow Cytometry

1-2 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml
Application Notes
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: E4F1

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Alternate Names

E4F transcription factor 1p50E4F, E4FMGC99614, EC 6.3.2.-, p120E4F, Putative E3 ubiquitin-protein ligase E4F1, Transcription factor E4F, transcription factor E4F1

Gene Symbol

E4F1

UniProt

Q66K89 (Human)

Additional E4F1 Products

Product Documents for E4F1 Antibody (PCRP-E4F1-2D1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for E4F1 Antibody (PCRP-E4F1-2D1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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