Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all E4F1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 725 and the C-terminus (residue 784) of human E4F1 (p120E4F) using the numbering given in entry NP_004415.1 (GeneID 1877).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for E4F1 Antibody

Western Blot: E4F1 Antibody [NB100-2871]

Western Blot: E4F1 Antibody [NB100-2871]

Western Blot: E4F1 Antibody [NB100-2871] - Detection of human E4F1 by western blot and immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-E4F1 antibody NB100-2871 (lot NB100-2871-3) used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. E4F1 was also immunoprecipitated by a previous lot (NB100-2871-2) of this antibody. Detection: Chemiluminescence with exposure times of 3 minutes (A and B).

Applications for E4F1 Antibody

Application
Recommended Usage

Immunoprecipitation

2- 5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: E4F1

The zinc finger protein encoded by this gene is one of several cellular transcription factors whose DNA-binding activities are regulated through the action of adenovirus E1A. A 50-kDa amino-terminal product is generated from the full-length protein through proteolytic cleavage. The protein is differentially regulated by E1A-induced phosphorylation. The full-length gene product represses transcription from the E4 promoter in the absence of E1A, while the 50-kDa form acts as a transcriptional activator in its presence.

Alternate Names

E4F transcription factor 1p50E4F, E4FMGC99614, EC 6.3.2.-, p120E4F, Putative E3 ubiquitin-protein ligase E4F1, Transcription factor E4F, transcription factor E4F1

Entrez Gene IDs

1877 (Human)

Gene Symbol

E4F1

UniProt

Q66K89 (Human)

Additional E4F1 Products

Product Documents for E4F1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for E4F1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.