EB2 Antibody (KT52)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23289
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # KT52
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Clone KT52 was generated from a GST fusion protein containing human EB2. This sequence is highly conserved in rat and mouse EB2. [UniProt# Q15555]
Specificity
The antibody detects a 36 kDa protein corresponding to the molecular mass of EB2 on SDS-PAGE immunoblots of human HeLa, mouse brain, and rat PC12 cells. The antibody also detects EB2 by immunocytochemistry in HeLa and A431 cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Scientific Data Images for EB2 Antibody (KT52)
Western Blot: EB2 Antibody (KT52) [NBP3-23289] -
Western Blot: EB2 Antibody (KT52) [NBP3-23289] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.
Immunocytochemistry/Immunofluorescence: EB2 Antibody (KT52) [NBP3-23289] -
Immunocytochemistry/Immunofluorescence: EB2 Antibody (KT52) [NBP3-23289] - Immunocytochemical labeling of EB2 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (left) and rat monoclonal anti-EB2 (NBP3-23289) (right). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat: DyLight(R) 594.Applications for EB2 Antibody (KT52)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EB2
Alternate Names
APC-binding protein EB2, EB1, EB2APC-binding protein EB1, End-binding protein 2, microtubule-associated protein, RP/EB family, member 2, RP1microtubule-associated protein RP/EB family member 2, T-cell activation protein, EB1 family
Gene Symbol
MAPRE2
Additional EB2 Products
Product Documents for EB2 Antibody (KT52)
Product Specific Notices for EB2 Antibody (KT52)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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