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EB2 Antibody (OTI1F3)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-00714

Novus Biologicals, part of Bio-Techne
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NBP2-00714

Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # OTI1F3

Concentration

0.81 mg/ml

Product Specifications

Immunogen

Full length human recombinant protein of human MAPRE2 (NP_055083) produced in HEK293T cell.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

36.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EB2 Antibody (OTI1F3)

Western Blot: EB2 Antibody (OTI1F3) [NBP2-00714]

Western Blot: EB2 Antibody (OTI1F3) [NBP2-00714]

Western Blot: EB2 Antibody (1F3) [NBP2-00714] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EB2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-EB2.
Immunocytochemistry/Immunofluorescence: EB2 Antibody (1F3) [NBP2-00714] Staining of COS7 cells transiently transfected by pCMV6-ENTRY EB2.
Immunohistochemistry-Paraffin: EB2 Antibody (OTI1F3) [NBP2-00714]

Immunohistochemistry-Paraffin: EB2 Antibody (OTI1F3) [NBP2-00714]

Immunohistochemistry-Paraffin: EB2 Antibody (1F3) [NBP2-00714] - Staining of paraffin-embedded Human pancreas tissue using anti-EB2 mouse monoclonal antibody.

Applications for EB2 Antibody (OTI1F3)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

0.81 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EB2

The protein encoded by this gene shares significant homology to the adenomatous polyposis coli (APC) protein-binding EB1 gene family. The function of this protein is unknown; however, its homology suggests involvement in tumorigenesis of colorectal cancers and proliferative control of normal cells. This gene may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Alternative splicing results in multiple transcript variants. [provided by RefSeq].

Alternate Names

APC-binding protein EB2, EB1, EB2APC-binding protein EB1, End-binding protein 2, microtubule-associated protein, RP/EB family, member 2, RP1microtubule-associated protein RP/EB family member 2, T-cell activation protein, EB1 family

Entrez Gene IDs

10982 (Human)

Gene Symbol

MAPRE2

Additional EB2 Products

Product Documents for EB2 Antibody (OTI1F3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EB2 Antibody (OTI1F3)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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