EB3 Antibody (KT36)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23294
Key Product Details
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2A Clone # KT36
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for EB3 Antibody (KT36)
Immunogen
Clone KT36 was generated from a GST fusion protein containing human EB3. This sequence is highly conserved in rat and mouse EB3. [UniProt# Q9UPY8]
Specificity
The antibody detects a 32 kDa protein corresponding to the molecular mass of EB3 on SDS-PAGE immunoblots of human HeLa cells and mouse brain tissue. The antibody also detects EB3 by immunocytochemistry in HeLa and A431 cells.
Clonality
Monoclonal
Host
Rat
Isotype
IgG2A
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage).
Scientific Data Images for EB3 Antibody (KT36)
Western Blot: EB3 Antibody (KT36) [NBP3-23294] -
Western Blot: EB3 Antibody (KT36) [NBP3-23294] - Western blot of EB proteins in mouse brain (lanes 1-5). The blot was probed with rat monoclonals anti-EB1 (lane 1), anti-EB1/2/3 (lane 2), NBP3-23289 anti-EB2 (lane 3), NBP3-23293 anti-EB3 (lane 4), and NBP3-23294 anti-EB3 (lane 5). Then the antibodies were detected using goat anti-Rat IgG Light Chain specific: HRP.
Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] -
Immunocytochemistry/Immunofluorescence: EB3 Antibody (KT36) [NBP3-23294] - Immunocytochemical labeling of EB3 in paraformaldehyde-fixed and NP40-permeabilized A431 cells. The cells were dual labeled with mouse monoclonal anti-alpha Tubulin (NBP3-23258) (green) and rat monoclonal anti-EB3 (NBP3-23294) (red). The antibodies were detected using either goat anti-mouse: DyLight(R) 488 or goat anti-Rat:DyLight(R) 594.Applications for EB3 Antibody (KT36)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Preservative
0.05% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EB3
Alternate Names
EB1 protein family member 3, EB3EBF3-S, End-binding protein 3, microtubule-associated protein RP/EB family member 3, microtubule-associated protein, RP/EB family, member 3, RP3EBF3APC binding protein
Gene Symbol
MAPRE3
Additional EB3 Products
Product Documents for EB3 Antibody (KT36)
Product Specific Notices for EB3 Antibody (KT36)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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