EB3 [p Ser176] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-23300

Novus Biologicals, part of Bio-Techne
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NBP3-23300
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EB3 Antibodies »

Product Specifications

Immunogen

Phospho-EB3 (Ser-176) synthetic peptide (coupled to KLH) corresponding to amino acid residues surrounding serine 176 in human EB3. This peptide sequence is highly conserved in mouse and rat EB3. This site is conserved in EB2 but the neighboring amino acids are not well conserved. [UniProt# Q9UPY8]

Modification

p Ser176

Specificity

This antibody was cross-absorbed to unphospho-EB3 (Ser-176), before affinity purification using phospho-EB3 (Ser-176) peptide (without carrier). The antibody detects a 32 kDa protein corresponding to the apparent molecular mass of phosphorylated EB3 on SDS-PAGE immunoblots of mouse brain. This reactivity is significantly reduced after lambda phosphatase treatment.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Stable for at least 1 year at -20°C.

Scientific Data Images for EB3 [p Ser176] Antibody

EB3 [p Ser176] Antibody

Western Blot: EB3 [p Ser176] Antibody [NBP3-23300] -

Western Blot: EB3 [p Ser176] Antibody [NBP3-23300] - Western blot analysis of adult mouse brain untreated (lanes 1, 3, 5, & 7) or treated with lambda phosphatase (lanes 2, 4, 6, & 8). The blots were probed with rat monoclonal anti-EB3 (NBP3-23294) (lanes 1 & 2), and rabbit polyclonals anti-EB3 (Ser-162) (lanes 3 & 4), anti-EB3 (Ser-176) (lanes 5 & 6), and anti-EB3 (a.a. 171-182) (lanes 7 & 8).

Applications for EB3 [p Ser176] Antibody

Application
Recommended Usage

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1 mg/ml BSA and 50% glycerol

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: EB3

EB3 is a 282 amino acid homologue of EB1, a protein known to bind APC (adenomatous polyposis coli). It has been shown to bind to tubulin in vitro and microtubules in vivo. Northern blotting shows expressions to be mainly in brain, and to a lesser degree in muscle. EB3 interacts with APCL, a homologue of the tumor suppressor APC, and this interaction may be involved in the stabilization or extension of microtubules.

Alternate Names

EB1 protein family member 3, EB3EBF3-S, End-binding protein 3, microtubule-associated protein RP/EB family member 3, microtubule-associated protein, RP/EB family, member 3, RP3EBF3APC binding protein

Gene Symbol

MAPRE3

Additional EB3 Products

Product Documents for EB3 [p Ser176] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EB3 [p Ser176] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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