EBAG9/RCAS1 Antibody (CPTC-EBAG9-1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07534

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2c Kappa Clone # CPTC-EBAG9-1

Concentration

0.2 mg/ml

View all EBAG9/RCAS1 Antibodies »

Product Specifications

Immunogen

Recombinant human full-length EBAG9/RCAS1 protein (Uniprot: O00559)

Localization

Golgi apparatus

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2c Kappa

Theoretical MW

32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08859)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for EBAG9/RCAS1 Antibody (CPTC-EBAG9-1)

Immunocytochemistry/ Immunofluorescence: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534]

Immunocytochemistry/ Immunofluorescence: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534]

Immunocytochemistry/Immunofluorescence: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534] - Immunofluorescence Analysis of Human MCF-7 cells labeling EBAG9/RCAS1 with EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1) followed by goat anti- Mouse IgG-CF488 (Green). The nuclear counterstain is RedDot (Red).
Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody (CPTC-EBAG9-1) [NBP3-07534] - Formalin-fixed, paraffin-embedded human Unknown Tumor stained with EBAG9/RCAS1 Mouse Monoclonal Antibody (CPTC-EBAG9-1).

Applications for EBAG9/RCAS1 Antibody (CPTC-EBAG9-1)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: EBAG9/RCAS1

EBAG9 was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Alternate Names

Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells

Gene Symbol

EBAG9

UniProt

O00559 (Human)

Additional EBAG9/RCAS1 Products

Product Documents for EBAG9/RCAS1 Antibody (CPTC-EBAG9-1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EBAG9/RCAS1 Antibody (CPTC-EBAG9-1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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