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EBAG9/RCAS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47292

Novus Biologicals, part of Bio-Techne
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NBP1-47292

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 163 and 213 of human Receptor-binding Cancer Antigen Expressed on SiSo cells using the numbering given in entry NP_004206.1 (GeneID 9166).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EBAG9/RCAS1 Antibody

Western Blot: EBAG9/RCAS1 Antibody [NBP1-47292]

Western Blot: EBAG9/RCAS1 Antibody [NBP1-47292]

Western Blot: EBAG9/RCAS1 Antibody [NBP1-47292] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-RCAS1 antibody NBP1-47292 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. RCAS1 was also immunoprecipitated by rabbit anti-RCAS1 antibody NBP1-47291, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Applications for EBAG9/RCAS1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EBAG9/RCAS1

This gene was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene. [provided by RefSeq]

Alternate Names

Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells

Entrez Gene IDs

9166 (Human)

Gene Symbol

EBAG9

Additional EBAG9/RCAS1 Products

Product Documents for EBAG9/RCAS1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EBAG9/RCAS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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