EBAG9/RCAS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00242

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all EBAG9/RCAS1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human EBAG9/RCAS1 (Accession#: O00559; Arg28-Ser213)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for EBAG9/RCAS1 Antibody

Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242]

Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242]

Western Blot: EBAG9/RCAS1 Antibody [NBP3-00242] - Anti-EBAG9/RCAS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: A431 Whole Cell Lysate. Lane B: MCF7 Whole Cell Lysate. Lane C: THP1 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 24 kDa. Observed band size: 37 kDa
Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242] - Immunochemical staining of human EBAG9 in human heart with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242]

Immunohistochemistry-Paraffin: EBAG9/RCAS1 Antibody [NBP3-00242] - Immunochemical staining of human EBAG9/RCAS1 in human prostate with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).
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Applications for EBAG9/RCAS1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:1000-1:4000

Immunoprecipitation

1-4 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EBAG9/RCAS1

EBAG9 was identified as an estrogen-responsive gene. Regulation of transcription by estrogen is mediated by estrogen receptor which binds to the estrogen-responsive element (ERE) found in the 5'-flanking region of this gene. The encoded protein is a tumor-associated antigen that is expressed at high frequency in a variety of cancers. Two transcript variants differing in the 5' UTR, but encoding the same protein, have been identified for this gene.

Alternate Names

Cancer-associated surface antigen RCAS1, EB9cancer associated surface antigen, estrogen receptor binding site associated, antigen, 9, Estrogen receptor-binding fragment-associated gene 9 protein, RCAS1PDAF, receptor-binding cancer antigen expressed on SiSo cells

Gene Symbol

EBAG9

Additional EBAG9/RCAS1 Products

Product Documents for EBAG9/RCAS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EBAG9/RCAS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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