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EBF-1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-28471

Novus Biologicals, part of Bio-Techne
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NBP1-28471

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Peptide ELISA, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

Product Specifications

Immunogen

Peptide with sequence C-HNQLPALANTSV corresponding to internal region according to NP_076870.1.

Epitope

C terminus

Specificity

This antibody is expected not to cross-react with EBF2, EBF3 and EBF4.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for EBF-1 Antibody

Western Blot: EBF-1 Antibody [NBP1-28471]

Western Blot: EBF-1 Antibody [NBP1-28471]

Western Blot: EBF-1 Antibody [NBP1-28471] - Analysis of EBF1 in Human Adipose lysate (35ug protein in RIPA buffer) using this antibody at 0.3 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for EBF-1 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:64000

Western Blot

0.3 - 0.1 ug/ml
Application Notes
IP: An anonymous customer has reported successful from induced 3T3-L1 expressing FLAG-EBF1. WB: Approx. 75 kDa band observed in human adipose lysates (calculated MW of 64.5 kDa band according to NP_076870.1).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EBF-1

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (Bysimilarity)

Long Name

Early B-cell Factor 1

Alternate Names

COE1, EBF1, OE-1, OLF1

Entrez Gene IDs

1879 (Human); 13591 (Mouse); 116543 (Rat)

Gene Symbol

EBF1

Additional EBF-1 Products

Product Documents for EBF-1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EBF-1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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