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EBF-3 Antibody (8D6)

Novus Biologicals, part of Bio-Techne | Catalog # H00253738-M05

Novus Biologicals, part of Bio-Techne
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H00253738-M05

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse, Rat

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 8D6

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

EBF3 (NP_001005463, 381 a.a. ~ 480 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LYGMPHNNQEIILKRAADIAEALYSVPRNHNQIPTLGNNPAHTGMMGVNSFSSQLAVNVSETSQANDQVGYSRNTSSVSPRGYVPSSTPQQSNYNTVSTS

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 29180763).

Specificity

EBF3 (8D6)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for EBF-3 Antibody (8D6)

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05]

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05]

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05] - Analysis of EBF3 expression in IMR-32 (Cat # L008V1).
Immunohistochemistry-Paraffin: EBF-3 Antibody (8D6) [H00253738-M05]

Immunohistochemistry-Paraffin: EBF-3 Antibody (8D6) [H00253738-M05]

Immunohistochemistry-Paraffin: EBF-3 Antibody (8D6) [H00253738-M05] - Analysis of monoclonal antibody to EBF3 on formalin-fixed paraffin-embedded human lymph node. Antibody concentration 0.3 ug/ml
Western Blot: EBF-3 Antibody (8D6) [H00253738-M05]

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05]

Western Blot: EBF-3 Antibody (8D6) [H00253738-M05] - Analysis of EBF3 expression in NIH/3T3 (Cat # L018V1).

Applications for EBF-3 Antibody (8D6)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for ELISA and immunohistochemistry (paraffin).

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EBF-3

Transcriptional activator which recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3' (Bysimilarity)

Long Name

Early B-cell Factor 3

Alternate Names

COE3, EBF3, OE-2

Entrez Gene IDs

253738 (Human)

Gene Symbol

EBF3

OMIM

607407 (Human)

Additional EBF-3 Products

Product Documents for EBF-3 Antibody (8D6)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EBF-3 Antibody (8D6)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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