ECH1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00324

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human

Applications

ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ECH1 Antibodies »

Product Summary for ECH1 Antibody

Immunogen

Produced in rabbits immunized with purified, recombinant Human ECH1 (Uniprot#: Q13011; Thr34-Leu328)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ECH1 Antibody

Western Blot: ECH1 Antibody [NBP3-00324]

Western Blot: ECH1 Antibody [NBP3-00324]

Western Blot: ECH1 Antibody [NBP3-00324] - Anti-ECH1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 36 kDa. Observed band size: 34 kDa
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human liver(cirrhosis) with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections). Positive staining was localized to cytoplasm of hepatocyte
Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324]

Immunohistochemistry-Paraffin: ECH1 Antibody [NBP3-00324] - Immunochemical staining of human ECH1 in human prostate with rabbit polyclonal antibody (1:10000, formalin-fixed paraffin embedded sections).
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Applications for ECH1 Antibody

Application
Recommended Usage

ELISA

1:5000-1:10000

Immunohistochemistry-Paraffin

1:2500-1:10000

Immunoprecipitation

0.5-2 uL/mg of lysate

Western Blot

1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECH1

ECH1 encodes a member of the hydratase/isomerase superfamily. The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins. The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome. The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase. This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway. Expression of the rat gene is induced by peroxisome proliferators.

Alternate Names

delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial, delta3,5-delta2,4-dienoyl-CoA isomerase, dienoyl-CoA isomerase, EC 5.3.3.-, enoyl CoA hydratase 1, peroxisomal, enoyl Coenzyme A hydratase 1, peroxisomal, HPXEL, peroxisomal enoyl-CoA hydratase 1

Gene Symbol

ECH1

Additional ECH1 Products

Product Documents for ECH1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ECH1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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