ECHS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-87078

Novus Biologicals, part of Bio-Techne
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NBP1-87078
NBP1-87078-25ul
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Key Product Details

Validated by

Orthogonal Validation, Independent Antibodies

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all ECHS1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ECHS1 Antibody

Western Blot: ECHS1 Antibody [NBP1-87078]

Western Blot: ECHS1 Antibody [NBP1-87078]

Western Blot: ECHS1 Antibody [NBP1-87078] - Analysis using Anti-ECHS1 antibody NBP1-87078 (A) shows similar pattern to independent antibody NBP1-87079 (B).
Immunocytochemistry/ Immunofluorescence: ECHS1 Antibody [NBP1-87078]

Immunocytochemistry/ Immunofluorescence: ECHS1 Antibody [NBP1-87078]

Immunocytochemistry/Immunofluorescence: ECHS1 Antibody [NBP1-87078] - Immunofluorescent staining of human cell line U-2 OS shows localization to mitochondria.
Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078]

Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078]

Immunohistochemistry-Paraffin: ECHS1 Antibody [NBP1-87078] - Staining of human kidney.
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Applications for ECHS1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECHS1

ECHS1 is encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]

Alternate Names

EC 4.2.1, enoyl CoA hydratase, short chain, 1, mitochondrial, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial, Enoyl-CoA hydratase 1, enoyl-CoA hydratase, mitochondrial, SCEHEC 4.2.1.17, Short-chain enoyl-CoA hydratase

Gene Symbol

ECHS1

Additional ECHS1 Products

Product Documents for ECHS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ECHS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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