ECHS1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-96977

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all ECHS1 Antibodies »

Product Specifications

Immunogen

Produced in rabbits immunized with purified, recombinant Human ECHS1 (rh ECHS1; AAH08906.1; 28A-290Q)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for ECHS1 Antibody

Western Blot: ECHS1 Antibody [NBP2-96977]

Western Blot: ECHS1 Antibody [NBP2-96977]

Western Blot: ECHS1 Antibody [NBP2-96977] - Anti-ECHS1 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate. Lane B: Hela Whole Cell Lysate. Lane C: 293T Whole Cell Lysate. Lane D: MCF7 Whole Cell Lysate. Lysates/proteins at 30 ug per lane. Secondary Goat Anti- Rabbit IgG H&L (Dylight 800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 31 kDa (We are unsure as to the identity of these extra bands).
Immunoprecipitation: ECHS1 Antibody [NBP2-96977]

Immunoprecipitation: ECHS1 Antibody [NBP2-96977]

Immunoprecipitation: ECHS1 Antibody [NBP2-96977] - ECHS1 was immunoprecipitated using: Lane A: 0.5 mg 293T Whole Cell Lysate. Lane B: 0.5 mg HepG2 Whole Cell Lysate4 ul anti-ECHS1 rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein G. Primary antibody: Anti-ECHS1 rabbit polyclonal antibody, at 1:100 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size: 31 kDa. Observed band size: 31 kDa

Applications for ECHS1 Antibody

Application
Recommended Usage

Immunoprecipitation

4-8 uL/mg of lysate

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Antigen and protein A Affinity-purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECHS1

ECHS1 is encoded by this gene functions in the second step of the mitochondrial fatty acid beta-oxidation pathway. It catalyzes the hydration of 2-trans-enoyl-coenzyme A (CoA) intermediates to L-3-hydroxyacyl-CoAs. The gene product is a member of the hydratase/isomerase superfamily. It localizes to the mitochondrial matrix. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. [provided by RefSeq]

Alternate Names

EC 4.2.1, enoyl CoA hydratase, short chain, 1, mitochondrial, enoyl Coenzyme A hydratase, short chain, 1, mitochondrial, Enoyl-CoA hydratase 1, enoyl-CoA hydratase, mitochondrial, SCEHEC 4.2.1.17, Short-chain enoyl-CoA hydratase

Gene Symbol

ECHS1

Additional ECHS1 Products

Product Documents for ECHS1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ECHS1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.