ECM-1/Secretory Component P85 Antibody (ECM1/792)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-44397

Novus Biologicals, part of Bio-Techne
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NBP2-44397-0.02mg
NBP2-44397-0.1mg
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Key Product Details

Species Reactivity

Human, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # ECM1/792

Concentration

0.2 mg/ml

View all ECM1 Antibodies »

Product Specifications

Immunogen

Recombinant full-length human ECM-1/Secretory Component P85 protein (Uniprot: Q16610)

Localization

Cell surface and cytoplasmic

Specificity

This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-47804)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for ECM-1/Secretory Component P85 Antibody (ECM1/792)

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) [NBP2-44397]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) [NBP2-44397]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with ECM-1/Secretory Component P85 Mouse Monoclonal Antibody (ECM1/792).
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) [NBP2-44397]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (ECM1/792) [NBP2-44397]

Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (rECM1/792) [NBP2-44397] - Human Colon Carcinoma stained with IgA Secretory Component Monoclonal Antibody (ECM1/792).

Applications for ECM-1/Secretory Component P85 Antibody (ECM1/792)

Application
Recommended Usage

Flow Cytometry

0.5-1 ug/million cells

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunofluorescence

1 - 2 ug/ml

Immunohistochemistry

0.5-1 ug/ml

Immunohistochemistry-Paraffin

0.5 - 1.0 ug/ml
Application Notes
Immunohistology (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: ECM1

Examples of positive human tissues: secretory mucosa of gastrointestinal and respiratory tract, epithelia of salivary glands, endometrium, endocervix, kidney, prostate, mammary gland.

Long Name

Extracellular Matrix Protein 1

Alternate Names

ECM1, p85, Secretory Component P85

Gene Symbol

ECM1

UniProt

Q16610

Additional ECM1 Products

Product Documents for ECM-1/Secretory Component P85 Antibody (ECM1/792)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for ECM-1/Secretory Component P85 Antibody (ECM1/792)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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