ECM-1/Secretory Component P85 Antibody (SC05)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05760
![Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP3-05760]](https://resources.bio-techne.com/images/products/pIgR-Antibody-SC05-Immunohistochemistry-Paraffin-NBP3-05760-img0001.jpg "Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP3-05760]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Rat
Applications
Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 kappa Clone # SC05
Concentration
0.2 mg/ml
Product Summary for ECM-1/Secretory Component P85 Antibody (SC05)
Immunogen
Full length native protein (purified) corresponding to Human IgA ECM-1/Secretory Component P85. Affinity-purified secretory component from human colostrum. (Uniprot: Q16610 )
Localization
Cell surface and cytoplasmic
Specificity
This monoclonal antibody reacts with a reduction-resistant epitope present in both free and SIgA bound Secretory Component. It does not react with the cell lines lacking secretory component. The antibody is useful for studying the distribution and level of both free and bound secretory component. Secretory component is differentially expressed in epithelium, and the antibody is a popular marker for identifying subpopulations of epithelial cells and epithelial differentiation. The Secretory component antibody is a useful research tool for studying mucosal immunity, inflammation, remodeling, differentiation and tumorigenesis, all processes associated with differential secretory component expression.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-05762)
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
Scientific Data Images for ECM-1/Secretory Component P85 Antibody (SC05)
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP3-05760]
Immunohistochemistry-Paraffin: ECM-1/Secretory Component P85 Antibody (SC05) [NBP3-05760] - Formalin-fixed, paraffin-embedded human Colon Carcinoma.Applications for ECM-1/Secretory Component P85 Antibody (SC05)
Application
Recommended Usage
Flow Cytometry
0.5-1 ug/million cells
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunohistochemistry
1-2ug/ml
Immunohistochemistry-Paraffin
0.5-1 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 0.5-1ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: ECM1
Long Name
Extracellular Matrix Protein 1
Alternate Names
ECM1, p85, Secretory Component P85, Free Secretory Component (FSC) , pIgA-Secretory Component Complex, pIgM-Secretory Component Complex, Secretory Component (SC), sIgA-Secretory Component Complex
Gene Symbol
ECM1
UniProt
Additional ECM1 Products
Product Documents for ECM-1/Secretory Component P85 Antibody (SC05)
Product Specific Notices for ECM-1/Secretory Component P85 Antibody (SC05)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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