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ECM29 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-14153

Novus Biologicals, part of Bio-Techne
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NBP2-14153
NBP2-14153-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for ECM29 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: EDPKLLSMAYSAVGKLSSRMPHLFTKDIALVQQLFEALCKEEPETRLAIQEALSMMVGAYSTLEGAQRTLMEALVASYLIKPEVQVRQVAVKFASTVFPSDHIPSRYLLLLAAGDPREEVHGEAQRVLRCLP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ECM29 Antibody

Immunocytochemistry/ Immunofluorescence: ECM29 Antibody [NBP2-14153]

Immunocytochemistry/ Immunofluorescence: ECM29 Antibody [NBP2-14153]

Immunocytochemistry/Immunofluorescence: ECM29 Antibody [NBP2-14153] - Immunofluorescent staining of human cell line RH-30 shows localization to nucleus.
Immunohistochemistry-Paraffin: ECM29 Antibody [NBP2-14153]

Immunohistochemistry-Paraffin: ECM29 Antibody [NBP2-14153]

Immunohistochemistry-Paraffin: ECM29 Antibody [NBP2-14153] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry: ECM29 Antibody [NBP2-14153]

Immunohistochemistry: ECM29 Antibody [NBP2-14153]

Immunohistochemistry: ECM29 Antibody [NBP2-14153] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.

Applications for ECM29 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ECM29

Ecm29 appears to be one of three proteins which associate significantly with proteasomes. Ecm29 has been shown to enhance the stability of the proteasome. Ecm29 deletion has been shown to produce 26S proteasomes which dissociated in the absence of ATP. It was thus suggested that Ecm29 tethers the regulatory complex to the proteasome.

Alternate Names

Ecm29, ECM29 homolog, proteasome accessory protein, FLJ22036, homolog of yeast Ecm29, KIAA0368, KIAA1962, proteasome-associated protein ECM29 homolog

Gene Symbol

KIAA0368

Additional ECM29 Products

Product Documents for ECM29 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ECM29 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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