ECRG4 Antibody (OTI3B10) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-72294
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]](https://resources.bio-techne.com/images/products/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0003.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]")
![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]](https://resources.bio-techne.com/images/products/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0004.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]")
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
CyTOF-ready, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # OTI3B10
Format
Azide and BSA Free
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Full length human recombinant protein of human C2orf40 (NP_115787) produced in HEK293T cell.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for ECRG4 Antibody (OTI3B10) - Azide and BSA Free
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Staining of HeLa cells using anti-ECRG4 mouse monoclonal antibody.![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]](https://resources.bio-techne.com/images/products/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0001.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]")
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECRG4 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ECRG4.![Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]](https://resources.bio-techne.com/images/products/ECRG4-Antibody-OTI3B10-Azide-and-BSA-Free-Flow-Cytometry-NBP2-72294-img0002.jpg "Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]")
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294]
Flow Cytometry: ECRG4 Antibody (OTI3B10) - Azide and BSA Free [NBP2-72294] - Analysis of Hela cells, using anti-ECRG4 antibody, (Red), compared to a nonspecific negative control antibody (Blue).Applications for ECRG4 Antibody (OTI3B10) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1:100
Immunocytochemistry/ Immunofluorescence
1:100
Western Blot
1:500
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Reconstitution
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.
Formulation
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: ECRG4
Alternate Names
chromosome 2 open reading frame 40, ECRG4augurin, esophageal cancer related gene 4 protein, Esophageal cancer-related gene 4 protein
Gene Symbol
C2ORF40
Additional ECRG4 Products
Product Documents for ECRG4 Antibody (OTI3B10) - Azide and BSA Free
Product Specific Notices for ECRG4 Antibody (OTI3B10) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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