Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI2D9

Concentration

1 mg/ml

View all ECT2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 175-438 of human ECT2(NP_060568) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

99.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ECT2 Antibody (OTI2D9)

Western Blot: ECT2 Antibody (OTI2D9) [NBP2-46483]

Western Blot: ECT2 Antibody (OTI2D9) [NBP2-46483]

Western Blot: ECT2 Antibody (2D9) [NBP2-46483] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ECT2.
Immunohistochemistry: ECT2 Antibody (OTI2D9) [NBP2-46483]

Immunohistochemistry: ECT2 Antibody (OTI2D9) [NBP2-46483]

Immunohistochemistry: ECT2 Antibody (2D9) [NBP2-46483] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

Applications for ECT2 Antibody (OTI2D9)

Application
Recommended Usage

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Western Blot

1:2000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ECT2

Epithelial cell-transforming sequence 2 (ECT2) is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

Alternate Names

ARHGEF31, epithelial cell transforming sequence 2 oncogene, Epithelial cell-transforming sequence 2 oncogene, FLJ10461, MGC138291, protein ECT2

Gene Symbol

ECT2

Additional ECT2 Products

Product Documents for ECT2 Antibody (OTI2D9)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ECT2 Antibody (OTI2D9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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