Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all ECT2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 833 and 883 of human epithelial cell transforming sequence 2 using the numbering given in entry NP_060568.3 (GeneID 1894).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

104 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ECT2 Antibody

Western Blot: ECT2 Antibody [NBP1-28729]

Western Blot: ECT2 Antibody [NBP1-28729]

Western Blot: ECT2 Antibody [NBP1-28729] - Detection of Human and Mouse ECT2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-ECT2 antibody NBP1-28729 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: ECT2 Antibody [NBP1-28729]

Western Blot: ECT2 Antibody [NBP1-28729]

Western Blot: ECT2 Antibody [NBP1-28729] - Whole cell lysate (5, 15 and 50 ug) from HeLa cells. Antibody used for WB at 0.1 ug/ml.

Applications for ECT2 Antibody

Application
Recommended Usage

Western Blot

1:2000-1:10000
Application Notes
This antibody is not recommended for immunoprecipitation.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ECT2

Epithelial cell-transforming sequence 2 (ECT2) is a transforming protein that is related to Rho-specific exchange factors and yeast cell cycle regulators. The expression of this gene is elevated with the onset of DNA synthesis and remains elevated during G2 and M phases. In situ hybridization analysis showed that expression is at a high level in cells undergoing mitosis in regenerating liver. Thus, this protein is expressed in a cell cycle-dependent manner during liver regeneration, and is thought to have an important role in the regulation of cytokinesis.

Alternate Names

ARHGEF31, epithelial cell transforming sequence 2 oncogene, Epithelial cell-transforming sequence 2 oncogene, FLJ10461, MGC138291, protein ECT2

Entrez Gene IDs

1894 (Human)

Gene Symbol

ECT2

UniProt

Q9H8V3 (Human)

Additional ECT2 Products

Product Documents for ECT2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for ECT2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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