EDC4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-13944

Novus Biologicals, part of Bio-Techne
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NBP2-13944
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (98%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EDC4 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: VERALETRHEQEQRRLERALAEGQQRGGQLQEQLTQQLSQALSSAVAGRLERSIRDEIKKTVPPCVSRSLEPMAGQLSNSVATKLTAVEGSMKENISKLLKSKNLTDA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EDC4 Antibody

Western Blot: EDC4 Antibody [NBP2-13944]

Western Blot: EDC4 Antibody [NBP2-13944]

Western Blot: EDC4 Antibody [NBP2-13944] - Analysis in human cell line RT-4 and human cell line U-251 MG.
Immunocytochemistry/ Immunofluorescence: EDC4 Antibody [NBP2-13944]

Immunocytochemistry/ Immunofluorescence: EDC4 Antibody [NBP2-13944]

Immunocytochemistry/Immunofluorescence: EDC4 Antibody [NBP2-13944] - Staining of human cell line A-431 shows localization to nucleoplasm & cytoplasmic bodies.
Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-13944]

Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-13944]

Immunohistochemistry-Paraffin: EDC4 Antibody [NBP2-13944] - Staining of human Skeletal muscle shows very weak cytoplasmic positivity in myocytes.
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Applications for EDC4 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 -1:50

Immunohistochemistry-Paraffin

1:20-1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EDC4

In the process of mRNA degradation, seems to play a role in mRNA decapping. Component of a complex containing DCP2 and DCP1A which functions in decapping of ARE-containing mRNAs. Promotes complex formation between DCP1A and DCP2. Enhances the catalytic activity of DCP2 (in vitro)

Alternate Names

Autoantigen Ge-1, Autoantigen RCD-8, enhancer of mRNA decapping 4, enhancer of mRNA-decapping protein 4, Ge-1, Hedls, HEDLSHuman enhancer of decapping large subunit, RCD-8

Gene Symbol

EDC4

Additional EDC4 Products

Product Documents for EDC4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EDC4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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