eEF-2 Antibody (SR2299)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-21831

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-21831-100ul
NBP3-21831-50ul
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SR2299

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all eEF-2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human eEF-2 (Uniprot #: P13639)

Specificity

Detects endogenous levels of total eEF-2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

95 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eEF-2 Antibody (SR2299)

Western Blot: eEF-2 Antibody (SR2299) [NBP3-21831] -

Western Blot: eEF-2 Antibody (SR2299) [NBP3-21831] - Western blot analysis using NBP3-21831 in (1) A431 cell lysate; (2) NIH/3T3 cell lysate.

Applications for eEF-2 Antibody (SR2299)

Application
Recommended Usage

Flow Cytometry

1:50

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Immunoprecipitation

1:20

Western Blot

1:1000-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, 50% glycerol.

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: eEF-2

The eukaryotic translation Elongation Factor 2 (eEF2) is a 95 kDa member of the G-protein superfamily. Following peptide bond formation, eEF2 catalyzes translocation of the deacylated tRNA in the P-site and peptidyl tRNA in the A-site into the E- and P- sites, respectively (1). The activity of eEF2 is regulated by phosphorylation (2). To be active, eEF2 must be dephosphorylated, and phosphorylation at Thr-56 causes inactivation, resulting in the termination of mRNA translation (3). eEF2 is phosphorylated by a specific, calcium and calmodulin (Ca/CaM)-dependent eEF2 kinase (4).

Long Name

Eukaryotic Translation Elongation Factor 2

Alternate Names

eEF2

Gene Symbol

EEF2

Additional eEF-2 Products

Product Documents for eEF-2 Antibody (SR2299)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for eEF-2 Antibody (SR2299)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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