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EEF1A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-32555

Novus Biologicals, part of Bio-Techne
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NBP1-32555

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Predicted:

Bovine (100%), Chicken (99%), Rabbit (100%), Xenopus (98%), Zebrafish (95%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for EEF1A2 Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human EEF1A2. The exact sequence is proprietary.

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

50 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEF1A2 Antibody

Western Blot: EEF1A2 Antibody [NBP1-32555]

Western Blot: EEF1A2 Antibody [NBP1-32555]

Western Blot: EEF1A2 Antibody [NBP1-32555] - Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with EEF1A2 antibody [C1C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Western Blot: EEF1A2 Antibody [NBP1-32555]

Western Blot: EEF1A2 Antibody [NBP1-32555]

Western Blot: EEF1A2 Antibody [NBP1-32555] - Sample (50 ug of whole cell lysate) A: mouse brain 10% SDS PAGE; antibody diluted at 1:5000.
EEF1A2 Antibody

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP1-32555] -

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP1-32555] - EEF1A2 antibody [C1C3] detects EEF1A2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissue.
EEF1A2 stained by EEF1A2 antibody [C1C3] (NBP1-32555) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Applications for EEF1A2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:10000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EEF1A2

This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

eEF1A-2, EEF1ALFLJ41696, EF1A, EF-1-alpha-2, elongation factor 1-alpha 2, elongation factor-1 alpha, Eukaryotic elongation factor 1 A-2, eukaryotic translation elongation factor 1 alpha 2, HS1, statin, statin S1, statin-like, statin-S1, STN, STNL

Gene Symbol

EEF1A2

Additional EEF1A2 Products

Product Documents for EEF1A2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EEF1A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.