EEF1A2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-33280

Novus Biologicals, part of Bio-Techne
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NBP2-33280
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Predicted:

Mouse (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EEF1A2 Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: LLEALDTILPPTRPTDKPLRLPLQDVYKIGGIGTVPVGRVETGIL

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1A2 Antibody

Western Blot: EEF1A2 Antibody [NBP2-33280]

Western Blot: EEF1A2 Antibody [NBP2-33280]

Western Blot: EEF1A2 Antibody [NBP2-33280] - Analysis in human cell line RPMI-8226.
Immunocytochemistry/ Immunofluorescence: EEF1A2 Antibody [NBP2-33280]

Immunocytochemistry/ Immunofluorescence: EEF1A2 Antibody [NBP2-33280]

Immunocytochemistry/Immunofluorescence: EEF1A2 Antibody [NBP2-33280] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green
Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280]

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280]

Immunohistochemistry-Paraffin: EEF1A2 Antibody [NBP2-33280] - Staining of human skeletal muscle shows weak cytoplasmic positivity in a subset of myocytes.
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Applications for EEF1A2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 µg/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1A2

EEF1A2 encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 2) is expressed in brain, heart and skeletal muscle, and the other isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas. This gene may be critical in the development of ovarian cancer. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Alternate Names

eEF1A-2, EEF1ALFLJ41696, EF1A, EF-1-alpha-2, elongation factor 1-alpha 2, elongation factor-1 alpha, Eukaryotic elongation factor 1 A-2, eukaryotic translation elongation factor 1 alpha 2, HS1, statin, statin S1, statin-like, statin-S1, STN, STNL

Gene Symbol

EEF1A2

UniProt

Q05639 (Human)

Additional EEF1A2 Products

Product Documents for EEF1A2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EEF1A2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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