eEF1A2 binding protein Antibody (5B12)
Novus Biologicals, part of Bio-Techne | Catalog # H00091156-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # 5B12
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
DKFZp434B1231 (NP_840059, 720 a.a. ~ 818 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. GILPGHEYHFRVVAKNELGASKPSDTSQPWCIPRQRDRFTVKAPCYREPDLSQKPRFLVGLRSHLLPQGCECCMSCAVQGSPRPHVTWFKNDRSLEGNP
Reactivity Notes
Human. Other species not tested.
Specificity
DKFZp434B1231 - eEF1A2 binding protein
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Scientific Data Images for eEF1A2 binding protein Antibody (5B12)
![Western Blot: eEF1A2 binding protein Antibody (5B12) [H00091156-M01]](https://resources.bio-techne.com/images/products/eEF1A2-binding-protein-Antibody-5B12-Western-Blot-H00091156-M01-img0007.jpg "Western Blot: eEF1A2 binding protein Antibody (5B12) [H00091156-M01]")
Western Blot: eEF1A2 binding protein Antibody (5B12) [H00091156-M01]
Western Blot: eEF1A2 binding protein Antibody (5B12) [H00091156-M01] - DKFZp434B1231 monoclonal antibody (M01), clone 5B12 Analysis of DKFZp434B1231 expression in A-431.![Immunocytochemistry/ Immunofluorescence: eEF1A2 binding protein Antibody (5B12) [H00091156-M01]](https://resources.bio-techne.com/images/products/eEF1A2-binding-protein-Antibody-5B12-Immunocytochemistry-Immunofluorescence-H00091156-M01-img0005.jpg "Immunocytochemistry/ Immunofluorescence: eEF1A2 binding protein Antibody (5B12) [H00091156-M01]")
Immunocytochemistry/ Immunofluorescence: eEF1A2 binding protein Antibody (5B12) [H00091156-M01]
Immunocytochemistry/Immunofluorescence: eEF1A2 binding protein Antibody (5B12) [H00091156-M01] - Analysis of monoclonal antibody to DKFZp434B1231 on HeLa cell. Antibody concentration 15 ug/ml.
Sandwich ELISA: eEF1A2 binding protein Antibody (5B12) [H00091156-M01] - Detection limit for recombinant GST tagged DKFZp434B1231 is approximately 0.03ng/ml as a capture antibody.
Applications for eEF1A2 binding protein Antibody (5B12)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence and ELISA.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: eEF1A2 binding protein
Alternate Names
DKFZp434B1231, eEF1A2 binding protein, EEF1A2-binding protein 1, EEF1A2BP1, immunoglobulin-like and fibronectin type III domain containing 1, immunoglobulin-like and fibronectin type III domain-containing protein 1, KY-interacting protein 1, KYIP1
Entrez Gene IDs
91156 (Human)
Gene Symbol
IGFN1
Additional eEF1A2 binding protein Products
Product Documents for eEF1A2 binding protein Antibody (5B12)
Product Specific Notices for eEF1A2 binding protein Antibody (5B12)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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