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EEF1B2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-37875

Novus Biologicals, part of Bio-Techne
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NBP2-37875

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%), Rat (91%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: FGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATD

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1B2 Antibody

Western Blot: EEF1B2 Antibody [NBP2-37875]

Western Blot: EEF1B2 Antibody [NBP2-37875]

Western Blot: EEF1B2 Antibody [NBP2-37875] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG. Lane 4: Human Plasma. Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: EEF1B2 Antibody [NBP2-37875]

Immunocytochemistry/ Immunofluorescence: EEF1B2 Antibody [NBP2-37875]

Immunocytochemistry/Immunofluorescence: EEF1B2 Antibody [NBP2-37875] - Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoli fibrillar center & cytosol.
Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-37875]

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-37875]

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-37875] - Staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells.

Applications for EEF1B2 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1B2

EEF1B2 encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Alternate Names

EEF1B, EEF1B1, EF1B, EF-1-beta, elongation factor 1-beta, eukaryotic translation elongation factor 1 beta 1, eukaryotic translation elongation factor 1 beta 2

Gene Symbol

EEF1B2

UniProt

Additional EEF1B2 Products

Product Documents for EEF1B2 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EEF1B2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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