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EEF1B2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-92964

Novus Biologicals, part of Bio-Techne
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NBP2-92964-0.02ml
NBP2-92964-0.1ml

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Knockout Validated, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-225 of human EEF1B2 (NP_001032752.1). MGFGDLKSPAGLQVLNDYLADKSYIEGYVPSQADVAVFEAVSSPPPADLCHALRWYNHIKSYEKEKASLPGVKKALGKYGPADVEDTTGSGATDSKDDDDIDLFGSDDEEESEEAKRLREERLAQYESKKAKKPALVAKSSILLDVKPWDDETDMAKLEECVRSIQADGLVWGSSKLVPVGYGIKKLQIQCVVEDDKVGTDMLEEQITAFEDYVQSMDVAAFNKI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EEF1B2 Antibody - BSA Free

Western Blot: EEF1B2 AntibodyBSA Free [NBP2-92964]

Western Blot: EEF1B2 AntibodyBSA Free [NBP2-92964]

Western Blot: EEF1B2 Antibody [NBP2-92964] - Analysis of extracts of various cell lines, using EEF1B2 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.
Immunocytochemistry/ Immunofluorescence: EEF1B2 Antibody - BSA Free [NBP2-92964]

Immunocytochemistry/ Immunofluorescence: EEF1B2 Antibody - BSA Free [NBP2-92964]

Immunocytochemistry/Immunofluorescence: EEF1B2 Antibody [NBP2-92964] - Analysis of NIH/3T3 cells using EEF1B2 at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: EEF1B2 Antibody - BSA Free [NBP2-92964]

Immunohistochemistry-Paraffin: EEF1B2 Antibody - BSA Free [NBP2-92964]

Immunohistochemistry-Paraffin: EEF1B2 Antibody [NBP2-92964] - Paraffin-embedded rat spinal cord using EEF1B2 .

Applications for EEF1B2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:200

Immunohistochemistry

1:50-1:200

Western Blot

1:500-1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EEF1B2

EEF1B2 encodes a translation elongation factor. The protein is a guanine nucleotide exchange factor involved in the transfer of aminoacylated tRNAs to the ribosome. Alternative splicing results in three transcript variants which differ only in the 5' UTR.

Alternate Names

EEF1B, EEF1B1, EF1B, EF-1-beta, elongation factor 1-beta, eukaryotic translation elongation factor 1 beta 1, eukaryotic translation elongation factor 1 beta 2

Gene Symbol

EEF1B2

Additional EEF1B2 Products

Product Documents for EEF1B2 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EEF1B2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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