EEF1G Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-91862

Novus Biologicals, part of Bio-Techne
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NBP1-91862
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (99%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EEF1G Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: CNLITGMFQRLDKLRKNAFASVILFGTNNSSSISGVWVFRGQELAFPLSPDWQVDYESYTWRKLDPGSEETQTLVREYFSWEGAFQHVGKA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF1G Antibody

Immunohistochemistry-Paraffin: EEF1G Antibody [NBP1-91862]

Immunohistochemistry-Paraffin: EEF1G Antibody [NBP1-91862]

Immunohistochemistry-Paraffin: EEF1G Antibody [NBP1-91862] - Staining of human cerebellum, cerebral cortex, lymph node and testis using Anti-EEF1G antibody NBP1-91862 (A) shows similar protein distribution across tissues to independent antibody NBP2-68716 (B).
Western Blot: EEF1G Antibody [NBP1-91862]

Western Blot: EEF1G Antibody [NBP1-91862]

Western Blot: EEF1G Antibody [NBP1-91862] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: EEF1G Antibody [NBP1-91862]

Immunocytochemistry/ Immunofluorescence: EEF1G Antibody [NBP1-91862]

Immunocytochemistry/Immunofluorescence: EEF1G Antibody [NBP1-91862] - Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria. Antibody staining is shown in green.
View 4 more images

Applications for EEF1G Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EEF1G

EEF1G encodes a subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This subunit contains an N-terminal glutathione transferase domain, which may be involved in regulating the assembly of multisubunit complexes containing this elongation factor and aminoacyl-tRNA synthetases.

Alternate Names

eEF-1B gamma, EF-1-gamma, EF1Gelongation factor 1-gamma, eukaryotic translation elongation factor 1 gamma, GIG35, pancreatic tumor-related protein, PRO1608, translation elongation factor eEF-1 gamma chain

Gene Symbol

EEF1G

Additional EEF1G Products

Product Documents for EEF1G Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EEF1G Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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