Key Product Details

Species Reactivity

Validated:

Human, Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation (Negative), Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all EEF2K Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 675 and 725 of human calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase (EEF2K ) using the numbering given in entry NP_037434.1 (GeneID 29904).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EEF2K Antibody

Western Blot: EEF2K Antibody [NB100-87019]

Western Blot: EEF2K Antibody [NB100-87019]

Western Blot: EEF2K Antibody [NB100-87019] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB100-87019 used for WB at 0.4 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. eEF2 Kinase was successfully immunoprecipitated by NB100-87018, which recognizes an upstream epitope.
Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019]

Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019]

Immunohistochemistry-Paraffin: EEF2K Antibody [NB100-87019] - Section of human stomach carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-eEF2 Kinase used at a dilution of 1:1,000 (1ug/ml). Detection: DAB

Applications for EEF2K Antibody

Application
Recommended Usage

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. Immunoprecipitation Not recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EEF2K

This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment. [provided by RefSeq]

Alternate Names

Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EC 2.7.11, EC 2.7.11.20, eEF-2 kinase, eEF-2Kcalmodulin-dependent protein kinase III, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase, eukaryotic elongation factor 2 kinase, eukaryotic elongation factor-2 kinase, HSU93850, MGC45041

Entrez Gene IDs

29904 (Human)

Gene Symbol

EEF2K

UniProt

NP_037434.1 (Human)

Additional EEF2K Products

Product Documents for EEF2K Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EEF2K Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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