EEF2K Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12694
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for EEF2K Antibody
Immunogen
Produced in rabbits immunized with E. coli-derived Human EEF2K fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for EEF2K Antibody
![Western Blot: EEF2K Antibody [NBP3-12694]](https://resources.bio-techne.com/images/products/EEF2K-Antibody-Western-Blot-NBP3-12694-img0001.jpg "Western Blot: EEF2K Antibody [NBP3-12694]")
Western Blot: EEF2K Antibody [NBP3-12694]
Western Blot: EEF2K Antibody [NBP3-12694] - Lane A: A431 Whole Cell LysateLane B: H1299 Whole Cell LysateLane C: HeLa Whole Cell LysateLane D: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:82 kDa![Immunoprecipitation: EEF2K Antibody [NBP3-12694]](https://resources.bio-techne.com/images/products/EEF2K-Antibody-Immunoprecipitation-NBP3-12694-img0002.jpg "Immunoprecipitation: EEF2K Antibody [NBP3-12694]")
Immunoprecipitation: EEF2K Antibody [NBP3-12694]
Immunoprecipitation: EEF2K Antibody [NBP3-12694] - Lane A:0.5 mg HeLa Whole Cell Lysate4 uL anti-EEF2K rabbit polyclonal antibody and 60 ug of Immunomagnetic beads Protein A/G.Primary antibody:Anti-EEF2K rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilutionDeveloped using the ECL technique.Performed under reducing conditions.Predicted band size: 82 kDaObserved band size :100 kDaApplications for EEF2K Antibody
Application
Recommended Usage
Immunoprecipitation
1-5ul/mg of lysate
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: EEF2K
Alternate Names
Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase, calcium/calmodulin-dependent eukaryotic elongation factor-2 kinase, EC 2.7.11, EC 2.7.11.20, eEF-2 kinase, eEF-2Kcalmodulin-dependent protein kinase III, elongation factor-2 kinase, eukaroytic elongation factor 2 kinase, eukaryotic elongation factor 2 kinase, eukaryotic elongation factor-2 kinase, HSU93850, MGC45041
Gene Symbol
EEF2K
Additional EEF2K Products
Product Specific Notices for EEF2K Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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