Eg5 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-84888
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: EMILSPTVVSILKINSQLKHIFKTSLTVADKIEDQKKELDGFLSILCNNLHELQENTICSLVESQKQCGNLTEDLKTIKQTHSQELCKLMNLWTERFCALEEKCENIQKPLSSVQENIQQKSKDIVNKMTFHSQKFCADSDGFSQELR
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 25277178).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Eg5 Antibody
Western Blot: Eg5 Antibody [NBP1-84888]
Western Blot: Eg5 Antibody [NBP1-84888] - Analysis in control (vector only transfected HEK293T lysate) and KIF11 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84888]
Immunohistochemistry-Paraffin: Eg5 Antibody [NBP1-84888] - Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.Applications for Eg5 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
0.04-0.4 ug/ml
Application Notes
IHC reported in scientific literature (PMID: 25277178). For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Eg5
Long Name
Kinesin-like protein KIF11
Alternate Names
KIF11, KNSL1, TRIP-5, TRIP5
Gene Symbol
KIF11
Additional Eg5 Products
Product Documents for Eg5 Antibody
Product Specific Notices for Eg5 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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