Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1B12

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all EGR2 Antibodies »

Product Specifications

Immunogen

Human recombinant protein fragment corresponding to amino acids 156-476 of human EGR2(NP_000390) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

50.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EGR2 Antibody (OTI1B12) - Azide and BSA Free

Western Blot: EGR2 Antibody (OTI1B12)Azide and BSA Free [NBP2-70624]

Western Blot: EGR2 Antibody (OTI1B12)Azide and BSA Free [NBP2-70624]

Western Blot: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY EGR2.
Immunocytochemistry/ Immunofluorescence: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624]

Immunocytochemistry/ Immunofluorescence: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624]

Immunocytochemistry/Immunofluorescence: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of COS7 cells transiently transfected by pCMV6-ENTRY EGR2.
Immunohistochemistry: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624]

Immunohistochemistry: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624]

Immunohistochemistry: EGR2 Antibody (OTI1B12) - Azide and BSA Free [NBP2-70624] - Analysis of Adenocarcinoma of Human ovary tissue. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min)
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Applications for EGR2 Antibody (OTI1B12) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50-1:100

Immunohistochemistry

1:150

Western Blot

1:500

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: EGR2

Early Growth Response 2 (EGR2) is an early growth response family member which binds to specific areas of DNA and helps control the activity of particular genes. Specifically, EGR2 activates several genes that are involved in the formation and maintenance of myelin, the fatty substance that covers and protects nerve cells.

Defects in EGR2 have been linked to a number of diseases including congenital hypomyelination neuropathy, Charcot-Marie-Tooth disease type 1D, and Dejerine-Sottas neuropathy. EGR 2 antibodies may be useful tools for nerurscience research.

Alternate Names

AT591, CMT1D, CMT4E, early growth response 2, early growth response 2 (Krox-20 homolog, Drosophila), early growth response protein 2, EGR-2, FLJ14547, Krox-20 homolog, Drosophila, KROX-20, Drosophila, homolog (early growth response-2), KROX20DKFZp686J1957, Zinc finger protein Krox-20

Gene Symbol

EGR2

Additional EGR2 Products

Product Documents for EGR2 Antibody (OTI1B12) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for EGR2 Antibody (OTI1B12) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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