EGR2 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-92327

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all EGR2 Antibodies »

Product Specifications

Immunogen

Synthetic peptide made to an internal portion of the human EGR2 protein (between amino acids 370-420) [UniProt P11161].

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EGR2 Antibody - BSA Free

Western Blot: EGR2 AntibodyBSA Free [NB100-92327]

Western Blot: EGR2 AntibodyBSA Free [NB100-92327]

Western Blot: EGR2 Antibody [NB100-92327] - Analysis of pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:PC12 whole cell lysate(40ug) Lane3:NIH-3T3 whole cell lysate(40ug) Lane3:Jurkat whole cell lysate(40ug)
Immunocytochemistry/ Immunofluorescence: EGR2 Antibody - BSA Free [NB100-92327]

Immunocytochemistry/ Immunofluorescence: EGR2 Antibody - BSA Free [NB100-92327]

Immunocytochemistry/Immunofluorescence: EGR2 Antibody [NB100-92327] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-EGR2 at 5ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: EGR2 Antibody - BSA Free [NB100-92327]

Immunohistochemistry-Paraffin: EGR2 Antibody - BSA Free [NB100-92327]

Immunohistochemistry-Paraffin: EGR2 Antibody [NB100-92327] - IHC analysis of a formalin fixed and paraffin embedded tissue section of mouse brain using EGR2 antibody at 1:200 dilution. The signal was detected using HRP-conjugated anti-rabbit secondary antibody and DAB based detection reagent, and the sections were further counterstained with hematoxylin. This EGR2 antibody generated primarily a nuclear staining signal in the neurons and the glial cells, however, some cytoplasmic staining was also observed. The cytoplasmic signal potentially represents the translational or 14-3-3sigma interacting cytoplasmic pool of EGR2.
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Applications for EGR2 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

5 - 10 ug/ml

Immunohistochemistry

2 - 5 ug/ml

Immunohistochemistry-Paraffin

2 - 5 ug/ml

Western Blot

1 - 2 ug/ml
Application Notes
The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reviewed Applications

Read 1 review rated 1 using NB100-92327 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EGR2

Egr proteins function in transcription regulatory activities surrounding cellular growth, differentiation and function. The deduced amino acid sequences of human Egr-2 and mouse Egr-1 are 92% identical in the zinc finger region but show no homology elsewhere. Egr-2 is a sequence-specific DNA-binding transcription factor that binds two specific DNA sites located in the promoter region of HoxA4 and localizes to the nucleus. Defects in the Egr-2 protein are a cause of congenital hypomyelination neuropathy (CHN). CHN is characterized clinically by early onset of hypotonia, areflexia, distal muscle weakness and very slow nerve conduction velocities. Mutations in the gene that encodes Egr-2 (EGR2) also cause of Dejerine-Sottas syndrome (DSS), which is also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS patients exhibit severe early onset motor and sensory neuropathy with very slow nerve conduction velocities and elevated cerebrospinal fluid protein concentrations.

Alternate Names

AT591, CMT1D, CMT4E, early growth response 2, early growth response 2 (Krox-20 homolog, Drosophila), early growth response protein 2, EGR-2, FLJ14547, Krox-20 homolog, Drosophila, KROX-20, Drosophila, homolog (early growth response-2), KROX20DKFZp686J1957, Zinc finger protein Krox-20

Entrez Gene IDs

1959 (Human); 114090 (Rat)

Gene Symbol

EGR2

UniProt

P11161 (Human)

Additional EGR2 Products

Product Documents for EGR2 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EGR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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