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EHD3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31894

Novus Biologicals, part of Bio-Techne
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NBP2-31894

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (96%), Rat (96%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: KLYKSKLLPLEEHYRFHEFHSPALEDADFDNKPMVLLVGQYSTGKTTFIRY

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EHD3 Antibody

Western Blot: EHD3 Antibody [NBP2-31894]

Western Blot: EHD3 Antibody [NBP2-31894]

Western Blot: EHD3 Antibody [NBP2-31894] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31894]

Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31894]

Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31894] - Staining of human kidney shows strong positivity in glomeruli.
EHD3 Antibody

Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31894] -

Immunohistochemistry-Paraffin: EHD3 Antibody [NBP2-31894] - Staining of human placenta shows moderate membranous and cytoplasmic positivity in trophoblastic cells.

Applications for EHD3 Antibody

Application
Recommended Usage

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EHD3

The deduced protein identities between EHD1 and EHD2, between EHD1 and EHD3, and between EHD1 and EHD4, are 71%, 86%, and 76%, respectively. All contain multiple conserved regions that include a nucleotide binding consensus site at the N terminus, a bipartite nuclear localization signal, and an eps15 homology (EH) protein binding domain with an EF hand motif at the C terminus.

Alternate Names

EH domain containing 3, EH domain-containing protein 3, EHD2, EH-domain containing 3, PAST homolog 3, PAST3

Gene Symbol

EHD3

UniProt

Additional EHD3 Products

Product Documents for EHD3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EHD3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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