EHD4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-54873

Novus Biologicals, part of Bio-Techne
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NBP1-54873
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Cited:

Rat

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all EHD4 Antibodies »

Product Specifications

Immunogen

Synthetic peptides corresponding to EHD4(EH-domain containing 4) The peptide sequence was selected from the middle region of EHD4. Peptide sequence LMNLISQEETSTPTQLVQGGAFDGTTEGPFNQGYGEGAKEGADEEEWVVA. The peptide sequence for this immunogen was taken from within the described region.

Reactivity Notes

Use in Rat reported in scientific literature (PMID:30135925).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

61 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.

Scientific Data Images for EHD4 Antibody

Western Blot: EHD4 Antibody [NBP1-54873]

Western Blot: EHD4 Antibody [NBP1-54873]

Western Blot: EHD4 Antibody [NBP1-54873] - Trafficking proteins levels in hippocampus of 11 months old rats exposed to rotenone (ROT, 1 mg/kg/day) or DMSO (control), during 8 weeks, and exercised during 6 weeks (EXE) or left sedentary (SED). Representative images of immunoblot bands corresponding to SLP-1, Rab27B, CRPM-2, KIF1B, EDH-4, Dynactin, Dynein and beta-actin. Image collected and cropped by CiteAb from the following publication (https://linkinghub.elsevier.com/retrieve/pii/S2451830116300036) licensed under a CC-BY license.
Immunohistochemistry: EHD4 Antibody [NBP1-54873]

Immunohistochemistry: EHD4 Antibody [NBP1-54873]

Immunohistochemistry: EHD4 Antibody [NBP1-54873] - Human Thyroid lysate tissue at an antibody concentration of 5.0 ug/ml using anti-EHD4 antibody
Western Blot: EHD4 Antibody [NBP1-54873]

Western Blot: EHD4 Antibody [NBP1-54873]

Western Blot: EHD4 Antibody [NBP1-54873] - Reccomended Titration: 1 ug/ml Positive Control: Fetal Lung cell lysate
View 2 more images

Applications for EHD4 Antibody

Application
Recommended Usage

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1.0 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 2% Sucrose

Preservative

0.09% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EHD4

EHD4 is involved in the control of trafficking at the early endosome and regulates exit of cargo toward both the recycling compartment and the late endocytic pathway.

Alternate Names

EH domain containing 4, EH domain-containing protein 4, EH-domain containing 4, HCA10, HCA11, Hepatocellular carcinoma-associated protein 10/11, hepatocellular carcinoma-associated protein HCA11, ortholog of rat pincher, PAST homolog 4, PAST4

Gene Symbol

EHD4

UniProt

Q9H223 (Human)

Additional EHD4 Products

Product Documents for EHD4 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EHD4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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