eIF2 alpha/EIF2S1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-16295

Novus Biologicals, part of Bio-Techne
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NBP2-16295
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Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Xenopus (94%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all eIF2 alpha Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human eIF2 alpha/EIF2S1. The exact sequence is proprietary.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Xenopus laevis (94%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

36 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2 alpha/EIF2S1 Antibody

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-16295]

Western Blot: eIF2 alpha/EIF2S1 Antibody [NBP2-16295]

Western Blot: EIF2S1 Antibody [NBP2-16295] - Sample (30 ug of whole cell lysate) A: Hela 10% SDS PAGE gel, diluted at 1:2000.
Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NBP2-16295]

Immunohistochemistry-Paraffin: eIF2 alpha/EIF2S1 Antibody [NBP2-16295]

Immunohistochemistry-Paraffin: EIF2S1 Antibody [NBP2-16295] - Immunohistochemical analysis of paraffin-embedded MCF7 xenograft, using antibody at 1:100 dilution.

Applications for eIF2 alpha/EIF2S1 Antibody

Application
Recommended Usage

Immunohistochemistry

10 - 1:500

Immunohistochemistry-Paraffin

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

0.1M Tris (pH 7), 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: eIF2 alpha

The translation initiation factor eIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (36 kD), beta (38 kD, MIM 603908), and gamma (52 kD, MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM]

Long Name

Eukaryotic Translation Initiation Factor 2 Alpha

Alternate Names

EIF2S1

Gene Symbol

EIF2S1

UniProt

P05198

Additional eIF2 alpha Products

Product Documents for eIF2 alpha/EIF2S1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for eIF2 alpha/EIF2S1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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