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eIF2B epsilon Antibody (5M2D4)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16674

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-16674-100ul
NBP3-16674-20ul

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 5M2D4

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2B epsilon (Q13144). MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAA

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for eIF2B epsilon Antibody (5M2D4)

Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]

Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]

Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]

Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]

Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Immunofluorescence analysis of U-2 OS cells using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
eIF2B epsilon Antibody (5M2D4)

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -

Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for eIF2B epsilon Antibody (5M2D4)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: eIF2B epsilon

The eIF2B epsilon gene encodes one of five subunits of eukaryotic translation initiation factor 2B (EIF2B), a GTP exchange factor for eukaryotic initiation factor 2 and an essential regulator for protein synthesis. Mutations in this gene and the genes encoding other E

Alternate Names

CACH, CLE, EIF-2B, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2BE, EIF2Bepsilon, eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD), eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, LVWM, translation initiation factor eIF-2B subunit epsilon

Gene Symbol

EIF2B5

Additional eIF2B epsilon Products

Product Documents for eIF2B epsilon Antibody (5M2D4)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for eIF2B epsilon Antibody (5M2D4)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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