eIF2B epsilon Antibody (5M2D4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16674
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 5M2D4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human eIF2B epsilon (Q13144). MAAPVVAPPGVVVSRANKRSGAGPGGSGGGGARGAEEEPPPPLQAVLVADSFDRRFFPISKDQPRVLLPLANVALIDYTLEFLTATGVQETFVFCCWKAA
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
80 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for eIF2B epsilon Antibody (5M2D4)
Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]
Western Blot: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Western blot analysis of extracts of various cell lines, using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.Immunocytochemistry/ Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674]
Immunocytochemistry/Immunofluorescence: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Immunofluorescence analysis of U-2 OS cells using eIF2B epsilon (EIF2B5) Rabbit mAb (NBP3-16674) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] -
Immunohistochemistry-Paraffin: eIF2B epsilon Antibody (5M2D4) [NBP3-16674] - Analysis of eIF2B epsilon (EIF2B5) in paraffin-embedded human tonsil tissue using eIF2B epsilon (EIF2B5) Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.Applications for eIF2B epsilon Antibody (5M2D4)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:50 - 1:200
Western Blot
1:500 - 1:2000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: eIF2B epsilon
Alternate Names
CACH, CLE, EIF-2B, eIF-2B GDP-GTP exchange factor subunit epsilon, EIF2BE, EIF2Bepsilon, eukaryotic translation initiation factor 2B, subunit 5 (epsilon, 82kD), eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa, LVWM, translation initiation factor eIF-2B subunit epsilon
Gene Symbol
EIF2B5
Additional eIF2B epsilon Products
Product Documents for eIF2B epsilon Antibody (5M2D4)
Product Specific Notices for eIF2B epsilon Antibody (5M2D4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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