EIF2B2 Antibody (5B12-E10)

Novus Biologicals, part of Bio-Techne | Catalog # H00008892-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 5B12-E10

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all EIF2B2 Antibodies »

Product Specifications

Immunogen

EIF2B2 (AAH00494, 1 a.a. ~ 351 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRTDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL

Specificity

EIF2B2 - eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for EIF2B2 Antibody (5B12-E10)

Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01]

Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01]

Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - EIF2B2 monoclonal antibody (M01), clone 5B12-E10 Analysis of EIF2B2 expression in K-562.
Immunohistochemistry-Paraffin: EIF2B2 Antibody (5B12-E10) [H00008892-M01]

Immunohistochemistry-Paraffin: EIF2B2 Antibody (5B12-E10) [H00008892-M01]

Immunohistochemistry-Paraffin: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - Analysis of monoclonal antibody to EIF2B2 on formalin-fixed paraffin-embedded human lymphoma tissue. Antibody concentration 5 ug/ml.
Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01]

Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01]

Western Blot: EIF2B2 Antibody (5B12-E10) [H00008892-M01] - EIF2B2 monoclonal antibody (M01), clone 5B12-E10. Analysis of EIF2B2 expression in HeLa.
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Applications for EIF2B2 Antibody (5B12-E10)

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Sandwich ELISA

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for IHC-P and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EIF2B2

Eukaryotic initiation factor-2B (EIF2B) is a GTP exchange protein essential for protein synthesis. It consists of alpha (EIF2B1; MIM 606686), beta (EIF2B2), gamma (EIF2B3; MIM 606273), delta (EIF2B4; MIM 606687), and epsilon (EIF2B5; MIM 603945) subunits. EIF2B activates its EIF2 (see MIM 603907) substrate by exchanging EIF2-bound GDP for GTP.[supplied by OMIM]

Alternate Names

EIF2B, eIF-2B GDP-GTP exchange factor subunit beta, EIF2BB, EIF-2Bbeta, eukaryotic translation initiation factor 2B, subunit 2 (beta, 39kD), eukaryotic translation initiation factor 2B, subunit 2 beta, 39kDa, S20I15, S20III15, translation initiation factor eIF-2B subunit beta

Entrez Gene IDs

8892 (Human)

Gene Symbol

EIF2B2

OMIM

603 (Human)

UniProt

AAH00494 (Human)

Additional EIF2B2 Products

Product Documents for EIF2B2 Antibody (5B12-E10)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for EIF2B2 Antibody (5B12-E10)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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