eIF2B4 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-24813
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for eIF2B4 Antibody
Immunogen
This antibody has been engineered to specifically recognize the recombinant protein eIF2B4 using the following amino acid sequence: VGREMTKEEKLQLRKEKKQQKKKRKEEKGAEPETGSAVSAAQCQVGPTRELPESGIQLGTPREKVPAGRSKAELRAER
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for eIF2B4 Antibody
![eIF2B4 Antibody Western Blot: eIF2B4 Antibody [NBP3-24813]](https://resources.bio-techne.com/images/products/nbp3-24813_rabbit-eif2b4-pab-19122023169540.jpg "Western Blot: eIF2B4 Antibody [NBP3-24813]")
Western Blot: eIF2B4 Antibody [NBP3-24813]
Analysis in human cell line RT-4 and human cell line U-251 MG.![eIF2B4 Antibody Immunocytochemistry/Immunofluorescence: eIF2B4 Antibody [NBP3-24813]](https://resources.bio-techne.com/images/products/nbp3-24813_rabbit-eif2b4-pab-1912202316102311.jpg "Immunocytochemistry/Immunofluorescence: eIF2B4 Antibody [NBP3-24813]")
Immunocytochemistry/Immunofluorescence: eIF2B4 Antibody [NBP3-24813]
Staining of human cell line Hep-G2 shows localization to endoplasmic reticulum.![eIF2B4 Antibody Immunohistochemistry-Paraffin: eIF2B4 Antibody [NBP3-24813]](https://resources.bio-techne.com/images/products/nbp3-24813_rabbit-eif2b4-pab-2012202314593070.jpg "Immunohistochemistry-Paraffin: eIF2B4 Antibody [NBP3-24813]")
Immunohistochemistry-Paraffin: eIF2B4 Antibody [NBP3-24813]
Staining of human testis shows strong cytoplasmic positivity in cells in seminiferous ducts.Applications for eIF2B4 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 µg/ml
Immunohistochemistry
1:500 - 1:1000
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
0.04-0.4 µg/ml
Application Notes
For ICC/IF, we recommend using a combination of PFA and Triton X-100. This will give you the optimal results for your experiments. For IHC-Paraffin, we recommend using Heat-Induced Epitope Retrieval (HIER) with a pH level of 6. This optimized retrieval method ensures the best results for your experiments.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.2, 40% glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: eIF2B4
Long Name
Translation initiation factor eIF-2B subunit delta
Alternate Names
DKFZP586J0119, EIF2BD, EIF2Bdelta
Gene Symbol
EIF2B4
Additional eIF2B4 Products
Product Documents for eIF2B4 Antibody
Product Specific Notices for eIF2B4 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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