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EIF3B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38597

Novus Biologicals, part of Bio-Techne
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NBP2-38597
NBP2-38597-25ul

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (97%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: PPTLLSQEQIKQIKKDLKKYSKIFEQKDRLSQSKASKELVERRRTMMEDFRKYRKMAQELYMEQKNERLELRGGVDTDELDSNVDDWE

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF3B Antibody

Immunocytochemistry/ Immunofluorescence: EIF3B Antibody [NBP2-38597]

Immunocytochemistry/ Immunofluorescence: EIF3B Antibody [NBP2-38597]

Immunocytochemistry/Immunofluorescence: EIF3B Antibody [NBP2-38597] - Staining of human cell line MCF7 shows localization to nucleoplasm & cytosol. Antibody staining is shown in green.
EIF3B Antibody

EIF3B Antibody [NBP2-38597] -Analysis in human cell line A-431.

EIF3B Antibody [NBP2-38597] -Analysis in human cell line A-431.
EIF3B Antibody

EIF3B Antibody [NBP2-38597] -Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

EIF3B Antibody [NBP2-38597] -Staining of human testis shows moderate cytoplasmic positivity in cells in seminiferous ducts.

Applications for EIF3B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: EIF3B

Eukaryotic initiation factor 3 subunit B (eIF3B) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3B is a conserved subunit and part of the functional core of eIF3. It bears a non-canonical RNA recognition motif (RRM) and interacts directly with the eIF3S1 subunit.

Alternate Names

eIF3 p110, eIF3 p116, eIF3b, eIF-3-eta, EIF3-P110, EIF3-P116, EIF3S9EIF3-ETA, Eukaryotic translation initiation factor 3 subunit 9, eukaryotic translation initiation factor 3 subunit B, eukaryotic translation initiation factor 3, subunit 9 (eta, 116kD), eukaryotic translation initiation factor 3, subunit 9 eta, 116kDa, eukaryotic translation initiation factor 3, subunit B, MGC104664, MGC131875, Prt1 homolog, PRT1hPrt1

Gene Symbol

EIF3B

UniProt

Additional EIF3B Products

Product Documents for EIF3B Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for EIF3B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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