EIF3G Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-93298

Novus Biologicals, part of Bio-Techne
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NB100-93298
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Bovine (100%), Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunoprecipitation, Proximity Ligation Assay, Western Blot

Cited:

Proximity Ligation Assay, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all EIF3G Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 175 and 225 of human eukaryotic translation initiation factor 3, subunit G (eukaryotic translation initiation factor 3, subunit 4) using the numbering given in entry NP_003746.2 (G

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 25349259).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for EIF3G Antibody

Western Blot: EIF3G Antibody [NB100-93298]

Western Blot: EIF3G Antibody [NB100-93298]

Western Blot: EIF3G Antibody [NB100-93298] - Detection of human eIF3G/EIF3S4 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-eIF3G/EIF3S4 antibody NB100-93298 used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. eIF3G/EIF3S4 was also immunoprecipitated by two other rabbit anti-eIF3G/EIF3S4 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 3 seconds (B).

Applications for EIF3G Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in PLA reported in scientific literature (PMID:34260917).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: EIF3G

Eukaryotic initiation factor 3 subunit G (eIF3G) is one of at least 13 non-identical protein subunits of eukaryotic initiation factor 3 (eIF3). eIF3 is the largest eIF (~650 kDa) and functions to facilitate binding of the 40S ribosomal subunit to the 5'-end of cellular mRNAs near the cap structure (m7GpppN). eIF3G has been shown to directly interact with AIF (apoptosis inducing factor), a protein that plays a role in caspase-independent apoptosis. AIF is able to inhibit protein synthesis via its interaction with eIF3g.

Alternate Names

eIF3 p42, eIF3 p44, eIF-3-delta, eIF3-delta, eIF3g, EIF3-P42, eIF3-p44, EIF3S4eIF-3 RNA-binding subunit, Eukaryotic translation initiation factor 3 RNA-binding subunit, Eukaryotic translation initiation factor 3 subunit 4, eukaryotic translation initiation factor 3 subunit G, eukaryotic translation initiation factor 3 subunit p42, eukaryotic translation initiation factor 3, subunit 4 (delta, 44kD), eukaryotic translation initiation factor 3, subunit 4 delta, 44kDa, eukaryotic translation initiation factor 3, subunit G

Entrez Gene IDs

8666 (Human)

Gene Symbol

EIF3G

UniProt

O75821 (Human)

Additional EIF3G Products

Product Documents for EIF3G Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for EIF3G Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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